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Enzyme Panel

Biocatalysis is the fastest growing technology for synthesis of chiral molecules. Quantumzyme’s biocatalysis offerings comprises of redox enzymes such as

Redox Enzymes

ELGA

reductive amination enzymes such as Transaminases (TAs)

ELGA

cycloaddition enzymes for Diels-Alder’s reactions

Redox Enzymes

Genei Labs

reductive amination enzymes such as Transaminases (TAs)

cycloaddition enzymes for Diels-Alder’s reactions

Gencrest
The enzymes in these panels have been selected for great activity on a wide selection of substrates and over wide range of pH, accommodating the different synthetic needs of our customers.
Table 1
Description Product Code Accompanying documentation Panel Contents*
Alcohol Dehydrogenase ADH-001 ADH Product guide Cofactor regeneration guide 6 ADHs
Transaminase TA-001 TAM product guide Cofactor regeneration guide 6 TAs
Diels-Alderase DA-001 DA product guide 6 DAs
Each panel is supplied with a product guide containing all the information needed to work with our enzyme including: Our team of experts will help you find the best solution and assist you with any aspects of using our panels successfully in your facility. To find out how to order or for more information on our enzyme range, visit Quantumzyme.com/enzyme-panels.

Alcohol Dehydrogenases

The enzymes in this panel have been selected for their activity on a wideselection of substrates and in a widerange of pH, to encounter different synthetic needs of our customers.
Enantiopure alcohols can be obtained using our ADHs. (R) and (S)-enantiomers of acetophenone and its derivatives can be accessed by using these ADHs as well as racemic mixtures.

This panel contains 6 alcohol dehydrogenase (ADH) enzymes for the reduction of ketones and aldehydes to the corresponding alcohols. The enzymes in this panel belong to different protein folds i.e., short chain ADHs, ketoreductases, both from prokaryotes and eukaryotes. Also included in the enzyme is metagenomic samples and engineered enzymes.
ADHs need a cofactor (NADH or NADPH) for reducing the carbonyl. To be able to use this cofactor in catalytic amounts, the oxidised cofactor has to be continuously reduced, this is called cofactor regeneration. The enzymes accept either or both NAD(P)H cofactors and their regeneration can be achieved by adding a cofactor regeneration enzyme (GDH or FDH, also provided in the kit) and its substrate (D-glucose or sodium formate, respectively). In some cases, the same ADH is able to regenerate the cofactor in presence of isopropanol.
Table 2
Enzyme class Enzyme Cofactor regeneration Optimum pH
Alcohol Dehydrogenase ADH-001 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase> ADH-002 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-003 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-004 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-005 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-006 GDH, FDH pH 7.5 - 8.0

Transaminases

The enzymes in this panel have been selected for their activity on a wideselection of substrates and in a widerange of pH, to encounter different synthetic needs of our customers.Aromatic and aliphatic primary amines can be obtained using our transaminases(TAs) enzymes for the formal reductive amination of ketone to the corresponding (R) or (S)-amine. The results will depend on the inherent enzyme substrate scope but also on the substrate properties.
In fact, if the amine moiety in the product molecule is stabilized by hydrogen bonds with other neighboring functionalities, the reaction will reach higher conversions because the concurrent deamination reaction is less favoured.

TAs require a PLP cofactor for catalysing the reaction. PLP binds in the active site of the transaminase and it is instrumental for catalysing the transfer of the amine from an amino donor to the substrate, hence forming the desired chiral amine.
This panel contains 6 Transaminase (TAs) enzymes for the conversion of amino donor to the desired chiral amine.
Table 3
Enzyme class Enzyme Cofactor regeneration Optimum pH
Transaminase TA -001 GDH, FDH pH 7.5 - 8.0
Transaminase TA-002 GDH, FDH pH 7.5 - 8.0
Transaminase TA-003 GDH, FDH pH 7.5 - 8.0
Transaminase TA-004 GDH, FDH pH 7.5 - 8.0
Transaminase TA-005 GDH, FDH pH 7.5 - 8.0
Transaminase TA-006 GDH, FDH pH 7.5 - 8.0
Full enzyme descriptions, reaction setup and work-up instructions, as well as our expert tips, are included with each kit. Please contact us with any questions regarding this kit or its contents and we'll be happy to help.
Each panel is supplied with a product guide containing all the information needed to work with our enzyme including: Our team of experts will help you find the best solution and assist you with any aspects of using our panels successfully in your facility. To find out how to order or for more information on our enzyme range, visit Quantumzyme.com/enzyme-panels.

Diels Alderase

Diels-Alderase is an enzyme carrying out C-C bond formation between the diene and alkene/alkyne leading to cycloaddition reaction to form a cyclohexene ring. It requires MQ* ions for its activity. It works efficiently in the pH range of 7.0 -7.4 and temperature of 25°C 30°C.

Quantumzyme has a collection of 6 Diels-Alderase enzymes which can be provided for testing. If any one of them demonstrates the desired conversion, the same enzyme can be engineered by Quantumzyme and scaled up further.
Table 4
Shipping format Enzyme purity Enzyme concentration / amount Pack size Shipping condition Storage
Liquid Crude 10 mg/mL 5 Dry ice 15
Liquid Purified 3 mg/mL 3 Dry ice 20
Freeze dried powder Crude 100 mg 1 Room Temperature 20
Freeze dried powder Purified 10 mg 1 Room Temperature 25