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Enzyme Panel

QZyme screening panel is an easy and rapid way to determine the feasibility of using our engineered enzymes in your labs. Each screening kit contains carefully selected engineered enzymes for enhanced activity, selectivity, substrate range, solvent and temperature stability. It is conveniently packaged with enough enzyme for several screens.



The enzymes in these panels have been selected for activity on a wide selection of substrates and over wide range of pH, accommodating the different synthetic needs of our customers.
Description Product Code Accompanying Documentaion Panel Contents
Alcohol Dehydrogenase ADH-001 ADH Product guide
Cofactor regeneration guide
6 ADHs
Transaminase TA-001 TAM product guide
Cofactor regeneration guide
6 TAs
Diels-Alderase DA-001 DA product guide 6 DAs

Each panel is supplied with a product guide containing all the information needed to work with our enzyme including:

  • - Panel description and reaction mechanism
  • - Enzyme overview and application table
  • - Reaction set up and work-up
  • - Useful tips and cofactor regeneration recommendations
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Alcohol Dehydrogenases

The enzymes in this panel have been selected for their activity on a wideselection of substrates and in a widerange of pH, to encounter different synthetic needs of our customers.

Enantiopure alcohols can be obtained using our ADHs. (R) and (S)-enantiomers of acetophenone and its derivatives can be accessed by using these ADHs as well as racemic mixtures.

This panel contains 6 alcohol dehydrogenase (ADH) enzymes for the reduction of ketones and aldehydes to the corresponding alcohols. The enzymes in this panel belong to different protein folds i.e., short chain ADHs, ketoreductases, both from prokaryotes and eukaryotes. Also included in the enzyme is metagenomic samples and engineered enzymes.
ADHs need a cofactor (NADH or NADPH) for reducing the carbonyl. To be able to use this cofactor in catalytic amounts, the oxidised cofactor has to be continuously reduced, this is called cofactor regeneration. The enzymes accept either or both NAD(P)H cofactors and their regeneration can be achieved by adding a cofactor regeneration enzyme (GDH or FDH, also provided in the kit) and its substrate (D-glucose or sodium formate, respectively). In some cases, the same ADH is able to regenerate the cofactor in presence of isopropanol.

Enzyme Class Enzyme Cofactor Regeneration Optimum pH
Alcohol Dehydrogenase ADH-001 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-002 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-003 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-004 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-005 GDH, FDH pH 7.5 - 8.0
Alcohol Dehydrogenase ADH-006 GDH, FDH pH 7.5 - 8.0


Transaminases

Aromatic and aliphatic primary amines can be obtained using our transaminases(TAs) enzymes for the formal reductive amination of ketone to the corresponding (R) or (S)-amine. The results will depend on the inherent enzyme substrate scope but also on the substrate properties.

In fact, if the amine moiety in the product molecule is stabilised by hydrogen bonds with other neighbouring functionalities, the reaction will reach higher conversions because the concurrent deamination reaction is less favoured.

TAs require a PLP cofactor for catalysing the reaction. PLP binds in the active site of the transaminase and it is instrumental for catalysing the transfer of the amine from an amino donor to the substrate, hence forming the desired chiral amine.

This panel contains 6 Transaminase (TAs) enzymes for the conversion of amino donor to the desired chiral amine.

Enzyme Class Enzyme Cofactor Regeneration Optimum pH
Transaminase TA-001 PLP pH 7.5 - 8.0
Transaminase TA-002 PLP pH 7.5 - 8.0
Transaminase TA-003 PLP pH 7.5 - 8.0
Transaminase TA-004 PLP pH 7.5 - 8.0
Transaminase TA-005 PLP pH 7.5 - 8.0
Transaminase TA-006 PLP pH 7.5 - 8.0


Diels Alderase

Diels-Alderase is an enzyme carrying out C-C bond formation between the diene and alkene/alkyne leading to cycloaddition reaction to form a cyclohexene ring. It requires Mg ions for its activity. It works efficiently in the pH range of 7.0 -7.4 and temperature of 25ºC 30ºC.



Quantumzyme has a collection of 6 Diels-Alderase enzymes which can be provided for testing. If any one of them demonstrates the desired conversion, the same enzyme can be engineered by Quantumzyme and scaled up further.

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